Accumax Vs. Trypsin: A Guide To Serine Proteases For Cell Dissociation, Culture, And Applications
Accumax, a subtilisin serine protease from Bacillus licheniformis, and Trypsin, a trypsin serine protease from bovine pancreas, share applications in cell detachment and primary cell culture. Accumax dissociates cells non-enzymatically, while Trypsin cleaves specific peptide bonds, causing cell damage. Accumax is less specific and less damaging than Trypsin. Trypsin requires EDTA for activity, while Accumax…