Dbco Click Chemistry: A Strain-Promoted Revelation For Bioorthogonal Labeling
DBCO click chemistry, a type of strain-promoted azide-alkyne cycloaddition (SPAAC), involves the use of dibenzocyclooctyne (DBCO) as a highly reactive dipolarophile. Unlike copper-catalyzed azide-alkyne cycloaddition (CuAAC), SPAAC relies on inherent strain to drive the cycloaddition reaction. DBCO-based SPAAC is particularly useful in bioorthogonal chemistry, where its rapid and specific reaction with azides allows for selective…