Golden Gate Assembly Protocol: Efficient Dna Fragment Assembly For Synthetic Biology
Golden Gate assembly protocol is a modular cloning technique that allows efficient assembly of DNA fragments using Type IIS restriction enzymes and ligase. This multi-step protocol involves designing compatible DNA overhangs, cleaving fragments, and ligating them in a one-pot reaction. It enables hierarchical construction, iterative assembly, and compatibility with various applications in gene expression studies, synthetic biology, and genome engineering.