Primary Human Hepatocytes: Unveiling Liver Function, Drug Metabolism, And Regenerative Medicine
Primary human hepatocytes are specialized liver cells isolated from human tissue through enzymatic digestion or mechanical dissociation. As essential in vitro models, they provide insights into drug metabolism, toxicity, and liver diseases. Their applications extend to regenerative medicine, aiding in liver regeneration through in vivo transplantation. Culturing these cells optimizes their function, while cryopreservation ensures storage and sharing. Despite limitations like tissue availability and donor variability, ethical considerations and tissue acquisition guidelines guide their use. Research continues to explore their potential and overcome these challenges.